ISOLASI DAN IDENTIFIKASI BAKTERI PENAMBAT NITROGEN UNTUK PEMBUATAN BIOFERTILIZER
Abstract
Nitrogen is a macro nutrient needed by plants. Generally, people use inorganic fertilizers to fulfill nitrogen nutrients in plants. The problem then is, the continuous use of synthetic nitrogen fertilizers has a direct negative impact on the soil and a derivative impact on human health. The use of microorganisms, in this case bacteria, to provide nitrogen to plants can be done by isolating it and making it a biological fertilizer agent. Nitrogen fixing bacteria was isolated on the land of the oil palm plantation of PT Astra Agro Lestari. The isolated nitrogen-fixing bacteria were then tested quantitatively for their ability to fix nitrogen. The bacteria with the highest nitrogen fixing ability were then identified by sequencing their DNA nucleotide bases so that the bacterial strains were identified. The result is that there are 13 bacteria that are able to fix nitrogen with the codes J1, J3, Q5, L1, L11, J31, D1, M6, M5, R1, P2, J4 and C7. The quantitative test shows that bacteria with code D1 are the best at fixing nitrogen in the form of NH4, namely 0.27 ppm. The results of D1 bacterial DNA nucleotide base sequencing showed that the putitive Bacillus aerius strain 24K with identical values and query cover reach
Downloads
References
Arora S, Patel P N, Vanza MJ, Rao, GG (2014) Isolation and characterization of endophytic bacteria colonizing halophyte and other salt tolerant plant species from coastal gujarat, Afr. J. Microbial, Res, 8, 1779-1788.
Houda N, Hanene C, Ines M, Myriam BS, Imen D, Abdennaceur H (2014) Isolation and characterization of microbial communities from a constructed wetlands system: A case study in Tunisia, African Journal of Microbiology Research, 8, 529-538.
Kaburuan R, Hapsoh, Gusma W (2014) Isolasi dan Karakterisasi Bakteri Penambat nitrogen non-simbiotik tanah gambut cagar biosfer giam siak kecil-bukit batu, Jurnal agroteknologi, vol. 5 no. 1, agustus 2014 : 35 – 39.
Kumari KA, Kumar KNR, Rao CHN (2014) Adverse Effects of Chemical Fertilizers and Pesticides on Human Health and Environment, Journal of Chemical and Pharmaceutical Sciences (150-151).
Lakitan B (2013) Dasar-Dasar Fisiologi Tumbuhan, Depok, PT Rajagrafindo Persada.
Lubis SS, Sardi A, Huslina F, Lisa M (2020) Isolasi dan Karakterisasi Bakteri Pengikat Nitrogen Tanah Gambut Hutan Dari Kecamatan Trumon Aceh Selatan, Jurnal Pendidikan dan Biologi, 12(2), 117-129. doi: 10.25134/quagga.v12i2.2794.
Ludden PW (2001) Nitrogenase complex, Encyclopedia of life sciences, USA, 1-8.
Munawar A (2011) Kesuburan tanah dan Nutrisi tanaman. Bogor, IPB Press
Margaretha SL (2015) Dampak Inovasi Teknologi Pemupukan Nitrogen Pada Tanaman Jagung Terhadap Ketersediaan Pakan Ditingkat Petani (Studi Kasus: Kabupaten Gowa), Diakses di http://balitsereal.litbang.pertanian.go.id/wp-content/uploads/2018/01 /15se87.pdf.
Mirsha DK, Kumar, HD, Jha M (1985) Effects of Some Carbon Sources on Growth and Nitrogen Fixation in the Cyanobacterium Nostoc linckia, Biologia Plantarum (Praha), 27, 1-5.
Nurlita H, Utomo S (2011) Potensi nitrifikasi oleh bakteri yang terdapat di laut aliran kali plumbon, laut aliran kali banjir kanal barat dan laut aliran kali banjir kanal timur. Jurnal Presipitasi, 8, 1-7.
Savci S (2012) An Agricultural Pollutant: Chemical Fertilizer. International Journal of Environmental Science and Development, Vol. 3, No. 1.
Steenhoudt O, Vanderleyden J (2000) Azospirillum, a free-living nitrogen-fixing bacterium closely associated with grasses: genetic, biochemical and ecological aspects, FEMS Microbiology Reviews 24, 487-506.
Wagner SC (2011) Biological Nitrogen Fixation. Nature Education Knowledge 3(10):15
Widiyawati I, Sugiyanta, Juneadi A, Widyastuti R (2014) Peran Bakteri Penambat Nitrogen untuk Mengurangi Dosis Pupuk Nitrogen Anorganik pada Padi Sawah. J.Agraon. Indonesia 42 (2) : 96-102.
Young P, Torrey JG (1986) A comparison of carbon source utilization for growth and nitrogenase activity in two Frankia isolates, CaN. J. Microbial, 32, 353-358.
Yu, SS, Latt ZK, Kyew EP, Lynn, TM (2011) Accumulation of ammonia in culture broth by wild-type nitrogen-fixing bacterium, Stenotrophomonas maltophilia., IJABPT, 2, 72-77.
Copyright (c) 2021 VIABEL: Jurnal Ilmiah Ilmu-Ilmu Pertanian
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Copyright on any article is retained by the author(s).
- The author grants the journal, right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work’s authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal’s published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
- The article and any associated published material is distributed under the Creative Commons Attribution-ShareAlike 4.0 International License
.png)
