ISOLASI DAN UJI KEMAMPUAN BAKTERIOFAG SEBAGAI AGENS PENGENDALI PENYAKIT LAYU BAKTERI (Ralstonia solanacearum) PADA TANAMAN TOMAT

  • Fery Abdul Choliq Universitas Brawijaya
  • Mintarto Martosudiro Universitas Brawijaya
  • Istiqomah Istiqomah Universitas Islam Darul'Ulum Lamongan
  • Muhammad Fanhash Nijami
Abstract views: 108 , PDF downloads: 93
Keywords: Bacteriophage, Ralstonia solanacearum, tomato

Abstract

Tomato is the top priority in the development of horticultural crops. The obstacle which mostly encountered is Ralstonia solanacearum pathogen attack. Bacteriophage can cause bacterial lysis after they they develop themselves inside the bacteria. The specific of the bacteriophage can provide result quickly, accurately, and efficiently so that it can be used as an alternative to control bacterial wilt disease R. solanacearum environmentally friendly. This study aims to find out the effectiveness of bacteriophage to control the bacterial wilt disease R. solanacearum. The testing method are plaque assay, bacteriophage infections test in various dilutions, bacteriophage infection test in a liquid medium, and transmission electron microscopy test. In the greenhouse scale testing using a Completely Randomized Design (CRD) with 6 treatments and 4 replications. Quantitative data were analyzed using analysis of variance 5% error level and followed by least significant different  test level of 5%. The results showed that bacteriophages can infect R. solanacearum. Bacteriophage can infect bacteria R. solanacearum indicated by the appearance of plaques in NA media, the declining value of absorbance spectophotometer, and can lyse bacterial cells from dilutions 10-1 to 10-9. The morphology of bacteriophages that infect R. solanacearum have hexagonal head structure and it have which is with a size of 200 nm. In the greenhouse scale showed that the symptoms of R. solanacearum appear at 29 days after inoculation. Application of bacteriophages can control R. solanacearum with lower percentage than the control treatment.

References

Addy HS, A. Askora, T. Kawasaki, M. Fujie, dan T. Yamada. 2012. Utilization of filamentous phage RSM3 to control bacterial wilt caused by Ralstonia solanacearum . Plant disease 96(8): 1204-1209.
Bicalho RC, T. Santos, R. Gilbert, LS. Caixeta, LM. Taixeira, Bicalho, dan
Machado. 2009. Susceptibility of Escherichia coli Isolated from uteri of postpartum dairy cows to antibiotic and environmental Bacteriophages. Part I: Isolation and lytic activity estimation of bacteriophages. J. Dairy Science. 93: 93-104.
Biratu KS, T. Selvaraj, T. Hunduma. 2013. In vitro Evaluation of Actinobacteria against Tomato Bacterial Wilt (Ralstonia solanacearum EF Smith) in West Showa, Ethiopia. J. Plant Pathol Microb. 4 (1).
BPS, 2018. Statistik Tanaman Sayuran dan Buah‐buahan Semusim Indonesia 2018. https://www.bps.go.id/publication/2019/10/07/9c5dede09c805bc38302ea1c/statistik-tanaman-sayuran-dan-buah---buahan-semusim-indonesia-2018.html. (Diakses 29 Maret 2020).
Buana EOGHN dan K. Wardani. 2014. Isolasi Bakteriofag Litik Sebagai Agen Biosanitasi Pada Proses Pelisisan Bakteri Pembentuk Biofilm. J. Pangan dan Agroindustri. Ind 2 (2): 36-42.
Clokie MRJ, AD. Millard, AV. Letarov, dan S. Heaphy. 2011. Phages in Nature. J. Bacteriofag 1 (1): 31-45.
Dahal D, D. Heintz, AV. Dorsselaer, HP. Braun, dan K. Wydra. 2009. Pathogenesis and stress related, as well as metabolic proteins are regulated in tomato stems infected with Ralstonia solanacearum. J. Plant Physiology and Biochemistry 47: 838–846.
Elphinstone JG. 2005. The Current Bacterial Wilt Situation: a global view. APS, Press, St. Paul, Minnesota, USA, pp. 9e28.
Frampton RA, C Taylor, AVH. Moreno, SB. Visnovsky, NK. Petty, AR. Pitman, PC. Fineran. 2014. Identification of Bacteriophages for Biocontrol of the Kiwifruit Canker Phytopathogen Pseudomonas syringae pv. Actinidiae. J. Applied and Environmental Microbiology 80(7).
Hagens S dan MJ. Loesner, 2007. Application of Bacteriophage for detection and control of foodborne pathogens. J. Application microbiology 76(3): 513-519.
Iriarte FB, B. Balogh, M. Momol, Smith, M. Wilson dan J. Jones. 2007. Factor Affecting Survival of Bacteriophage on Tomato Leaf Surface. J. Applied and environmental microbiology. 76(6): 1704-1711.
Istiqomah, I., Kusumawati, D.E., 2018. Pemanfaatan Bacillus subtilis dan Pseudomonas fluorescens dalam pengendalian hayati Ralstonia solanacearum penyebab penyakit layu bakteri pada tomat. J. Agro 5, 1–12.
Kawa D, T. Olszak, K. Danis, M. Skrobek, HW. Ackermann. 2014. A giant Pseudomonas phage from Poland. J. Virologi 159:567–572.
Kawasaki T, H. Satsuma, M. Fujie, S. Usami, dan T. Yamada. 2007. Monitoring of Phytopathogenic Ralstonia solanacearum Cells Using Green Fluorescent Protein-Expressing Plasmid Derived from Bacteriophage φRSS1. J. Bioscience and bioengineering. 104(6): 451–456.
Kersten JT, H. Huang, dan C. Allen. 2001. Ralstonia solanacearum Needs Motility for Invasive Virulence on Tomato. J. Bacterioogyl 183(12): 3597.
Maharina KE, LQ. Aini, dan T. Wardiyati. 2014. Aplikasi Agens Hayati dan Bahan Nabati Sebagai Pengendalian Layu Bakteri (Ralstonia solanacearum) Pada Budidaya Tanaman Tomat. J. Produksi tanaman. 1(6).
Manjunath, D. Agsar, Jagannath, Rangaswamy, SC. Rao, S. Anand dan Yogisha. 2014. Characterization and In Vitro Efficacy Studies Of Wide Host Range Lytic Bacteriophage Φdmec-1 Infecting Escherichia coli Isolated from Pyogenic Skin Infections. J. Trend in live science (2): 2319–4731.
Meng F, 2013. Ralstonia solanacearum Species Complex and Bacterial Wilt Disease. J. Bacteriol Parasitol 4:2.
Murugaiyan S, J.Y. Bae, J. Wu, S.D. Lee, H.Y. Um, H.K. Choi, E. Chung, J.-H. Lee dan S.-W. Lee. 2010. Characterization of Filamentous Bacteriophage PE226 Infecting Ralstonia solanacearum Strains. J. Applied Microbiology.
Nindita LO, dan AK. Wardani. 2013. Purifikasi Phage Cocktail Serta Spektrum Penghambatannya Terhadap Bakteri Penyebab Foodborne Disease. J. Teknologi Pertanian. Ind 14 (1): 47-56.
Resita SR, NF. Azizi, FES. Iriyanto, WC. Yuliasari, M. Kiptiyah, dan HS. Addy. 2014. Pengendalian Hayati Penyakit Hawar Bakteri Pada Tanaman Kedelai Dengan Menggunakan Bakteriofag. Prosiding seminar nasional. Bangkalan 18 Juni 2014.
Seleim MAA, a. Elyousr, A.Moneem, dan F. Saead. 2014. First Report of Bacterial Wilt Caused by Ralstonia solanacearum Biovar 2 Race 1 on Tomato in Egypt. J.Plant Pathol 30 (3):299-303.
Setiadi dan SF Nurulhuda. 2003. Kentang Varietas dan Pembudidayaan. Penebar Swadaya. Jakarta.
Snyder L, dan W. Champness. 2003. Lytic Bacteriophages: Genetic Analysis And Transduction. In: Molecular Genetics of Bacteria. Washington, DC: ASM Press, p: 231-274
Susianto G, MM. Farid, NR. Dhany, dan HS. Addy. 2014. Host Range for Bacteriophages that Infect Bacterial Blight Pathogen on Soybean. Procedia Environmental Sciences. 760 – 766.
Taj MK,. J. X. Ling, L. L. Bing, Z. Qi, I. Taj, T. M. Hassani, Z. Samreen dan W. Yunlin. 2014. Effect of Dilution, Temperature and pH on The Lysis Activity of T4 Phage Against E.coli BL21. J. Animal. Plant Sci 24(4):1252-1255.
Yamada T, T. Kawasaki, S. Nagata, A. Fujiwara A, S. Usami, M. Fujie. 2007. New bacteriophages that infect the phytopathogen Ralstonia solanacearum. J. Microbiology 153: 2630–2639.
Yamada T. 2012. Bacteriophage of Ralstonia solanacearum: Their diversity and utilization as bioconhtrol agents in agriculture. In Tech.
Zhang L, J. Xu, J. Xu, H. Zhang, L. He, dan J. Feng. 2014. TssB is essential for virulence and required for Type VI secretion system in Ralstonia solanacearum. J. Microbial Pathogenesis 74:1-7.
Published
2020-05-29
How to Cite
Abdul Choliq, F., Martosudiro, M., Istiqomah, I., & Fanhash Nijami, M. (2020). ISOLASI DAN UJI KEMAMPUAN BAKTERIOFAG SEBAGAI AGENS PENGENDALI PENYAKIT LAYU BAKTERI (Ralstonia solanacearum) PADA TANAMAN TOMAT. VIABEL: Jurnal Ilmiah Ilmu-Ilmu Pertanian, 14(1), 8-20. https://doi.org/10.35457/viabel.v14i1.996
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Articles